Journal article

Luminal Mitosis Drives Epithelial Cell Dispersal within the Branching Ureteric Bud

Adam Packard, Kylie Georgas, Odysse Michos, Paul Riccio, Cristina Cebrian, Alexander N Combes, Adler Ju, Anna Ferrer-Vaquer, Anna-Katerina Hadjantonakis, Hui Zong, Melissa H Little, Frank Costantini

DEVELOPMENTAL CELL | CELL PRESS | Published : 2013

Abstract

The ureteric bud is an epithelial tube that undergoes branching morphogenesis to form the renal collecting system. Although development of a normal kidney depends on proper ureteric bud morphogenesis, the cellular events underlying this process remain obscure. Here, we used time-lapse microscopy together with several genetic labeling methods to observe ureteric bud cell behaviors in developing mouse kidneys. We observed an unexpected cell behavior in the branching tips of the ureteric bud, which we term "mitosis-associated cell dispersal." Premitotic ureteric tip cells delaminate from the epithelium and divide within the lumen; although one daughter cell retains a basal process, allowing it ..

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Grants

Awarded by NIH


Awarded by NHMRC


Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT


Awarded by NATIONAL CANCER INSTITUTE


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

We thank Adam B. White and Theresa Swayne for help with confocal microscopy, James Lefevre for help with statistical analysis, and Carl Bates, Hideki Enomoto, Joerg Fehling, Liqun Luo, and Andrew McMahon for kindly providing mouse strains. M.H.L. is a National Health and Medical Research Council (NHMRC) Senior Principal Research Fellow. This work was supported by grants from the NIH to F.C. (5R01DK083289) and A.P. (1F32DK096782) and the NHMRC to M.H.L. (APP1002748).