Journal article

The fasciclin-like arabinogalactan protein family of Eucalyptus grandis contains members that impact wood biology and biomechanics

Colleen P MacMillan, Lynette Taylor, Yingdong Bi, Simon G Southerton, Rob Evans, Antanas Spokevicius

NEW PHYTOLOGIST | WILEY | Published : 2015

Abstract

Fasciclin-like arabinogalactan protein (FLA) families have been identified and characterised in key plant species, with some members exhibiting functional specialization. Here we identify the FLA family of Eucalyptus grandis, and investigate the roles of three single-FAS domain FLAs, with particular focus on secondary cell-wall formation and wood properties. We use various in-silico approaches to identify and characterise E. grandis genome FLAs, and perform phylogenetic comparisons with other species. For three key FLAs, we perform functional testing including promoter-reporter and overexpression transgenic approaches using eucalypts, poplar and tobacco. Of the 18 eucalypt FLAs identified, s..

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Grants

Funding Acknowledgements

The Australian Department of Agriculture is thanked for financial assistance for the ISSA study through the Young Innovators and Scientist Award granted to L.T. The China Scholarship Council and the Chinese Ministry of Education is thanked for a joint PhD student grant to Y.B. Dr Maarten Stapper is thanked for kindly providing the use of the Stem Strength Tester. Dr Ming-Bo Wang is gratefully acknowledged for his detailed advice on the leaf-disk tobacco transformation technique. We would like to thank Mr Julio Najera for his assistance with transformation, harvest and identification of transgenic sectors as part of the ISSA study. We would also like to thank Dr Andrew Merchant, Dr Josquin Tibbits and Dr Philippe Rigault for providing RNAseq data on the FLAs in leaf and stem tissue. We also thank Dr Rosemary White and anonymous reviewers for helpful and constructive comments on the manuscript.