Comparison of IFCC-calibrated HbA(1c) from laboratory and point of care testing systems

Susan E Manley; Laura J Hikin; Rachel A Round; Peter W Manning; Stephen D Luzio; Gareth J Dunseath; Peter G Nightingale; Irene M Stratton; Robert Cramb; Kenneth A Sikaris; Stephen CL Gough; Jonathan Webber

Journal article

Comparison of IFCC-calibrated HbA(1c) from laboratory and point of care testing systems

Susan E Manley, Laura J Hikin, Rachel A Round, Peter W Manning, Stephen D Luzio, Gareth J Dunseath, Peter G Nightingale, Irene M Stratton, Robert Cramb, Kenneth A Sikaris, Stephen CL Gough, Jonathan Webber

DIABETES RESEARCH AND CLINICAL PRACTICE | ELSEVIER IRELAND LTD | Published : 2014

DOI: 10.1016/j.diabres.2014.05.003

Abstract

OBJECTIVE: WHO, IDF and ADA recommend HbA(1c) ≥6.5% (48 mmol/mol) for diagnosis of diabetes with pre-diabetes 6.0% (42 mmol/mol) [WHO] or 5.7% (39 mmol/mol) [ADA] to 6.4% (47 mmol/mol). We have compared HbA(1c) from several methods for research relating glycaemic markers. RESEARCH DESIGN AND METHODS: HbA1c was measured in EDTA blood from 128 patients with diabetes on IE HPLC analysers (Bio-Rad Variant II NU, Menarini HA8160 and Tosoh G8), point of care systems, POCT, (A1cNow+ disposable cartridges and DCA 2000(®)+ analyser), affinity chromatography (Primus Ultra2) and the IFCC secondary reference method (Menarini HA8160 calibrated using IFCC SRM protocol). RESULTS: Median (IQ range) on IFCC ..

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