Determination of Local Conformational Stability in Fragment 96–133 of Bovine Growth Hormone By High-Resolution 1H NMR Spectroscopy

Abstract

The specific assignment of resonances in the 400-MHz nuclear magnetic resonance (NMR) spectrum of fragment 96–133 (All) of bovine growth hormone (bSt) is described. Assignments have been made with homonuclear two-dimensional techniques, in particular that of sequential resonance assignment. Complete assignments were possible for the spin systems of 16 residues out of a total of 38 and partial assignments for another 5. Assignment of resonances to either residue type or a class of residue was possible for a number of other spin systems. Analysis of the type of nuclear Overhauser effect (NOE) indicates that segments 96–110 and 130–133 are nonregular stable structures and that the segment 111–1..