Journal article

Expression of stem cell factor in the postnatal rat testis

M Munsie, S Schlatt, DM deKretser, KL Loveland

MOLECULAR REPRODUCTION AND DEVELOPMENT | WILEY-LISS | Published : 1997

Abstract

The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timing that coincides with the onset of spermatogonial proliferation. Immunohistochemical staining of testes showed that SCF protein was readily detected within Sertoli cells at 1-7 dpp and in the adult. Immunoreactive material was also detected within gonocytes and within Leydig cells at 1-7 dpp and in Leydig cells in the adult. Given the intense staining of Leydig cells by two dif..

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University of Melbourne Researchers