Conference Proceedings

Novel method for demonstrating nuclear contribution in mouse nuclear transfer

M Munsie, T Peura, A Michalska, A Trounson, P Mountford

REPRODUCTION FERTILITY AND DEVELOPMENT | C S I R O PUBLISHING | Published : 1998

Abstract

Confirmation of nuclear contribution is essential to all nuclear transfer experiments. Contribution is easily demonstrated in nuclear transfer progeny but more difficult to confirm in nuclear transfer embryos. The use of donor nuclei isolated from lacZ transgenic mice offers a clear and simple method to demonstrate contribution in nuclear transfer embryos and offspring. The unique line of transgenic mice (Zin40) used in this study displays nuclear localised lacZ expression in all cells, including embryonic blastomeres, and demonstrates distinctive blue nuclei when treated with X-gal substrate. This characteristic staining pattern provided an ideal marker for demonstrating nuclear contributio..

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University of Melbourne Researchers