Journal article
Paediatric leukaemia DNA methylation profiling using MBD enrichment and SOLiD sequencing on archival bone marrow smears
NCL Wong, GD Meredith, G Marnellos, M Dudas, M Parkinson-Bates, MS Halemba, Z Chatterton, J Maksimovic, DM Ashley, F Mechinaud, JM Craig, R Saffery
Gigascience | OXFORD UNIV PRESS | Published : 2015
Open access
Abstract
Background: Acute Lymphoblastic Leukaemia (ALL) is the most common cancer in children. Over the past four decades, research has advanced the treatment of this cancer from a less than 60% chance of survival to over 85% today. The causal molecular mechanisms remain unclear. Here, we performed sequencing-based genomic DNA methylation profiling of eight paediatric ALL patients using archived bone marrow smear microscope slides.Findings: SOLiD™ sequencing data was collected from Methyl-Binding Domain (MBD) enriched fractions of genomic DNA. The primary tumour and remission bone marrow sample was analysed from eight patients. Four patients relapsed and the relapsed tumour was analysed. Input and M..
View full abstractGrants
Awarded by Peak Computing Facility at the Victorian Life Sciences Computational Initiative (VLSCI)
Funding Acknowledgements
The authors would like to thank Mike Payne and Ivonne Petermann from Life Technologies for technical support. We also thank Dr Fernando Rosello (Monash University) for his assistance in running the LifeScope Alignments. NCLW was supported by the Leukaemia Foundation, My Room, Victorian Cancer Agency and the National Health and Medical Research Council. RS is supported by a Senior Research Fellowship, National Health and Medical Research Council. The Murdoch Childrens Research Institute is supported by the Victorian Government Operational and Infrastructure Support Grant. Computational analysis was performed on the Peak Computing Facility at the Victorian Life Sciences Computational Initiative (VLSCI) under project VR0002.