Journal article

Sequence of Age-Associated Changes to the Mouse Neuromuscular Junction and the Protective Effects of Voluntary Exercise

Anson Cheng, Marco Morsch, Yui Murata, Nazanin Ghazanfari, Stephen W Reddel, William D Phillips



Loss of connections between motor neurons and skeletal muscle fibers contribute to motor impairment in old age, but the sequence of age-associated changes that precede loss of the neuromuscular synapse remains uncertain. Here we determine changes in the size of neuromuscular synapses within the tibialis anterior muscle across the life span of C57BL/6J mice. Immunofluorescence, confocal microscopy and morphometry were used to measure the area occupied by nerve terminal synaptophysin staining and postsynaptic acetylcholine receptors at motor endplates of 2, 14, 19, 22, 25 and 28 month old mice. The key findings were: 1) At middle age (14-months) endplate acetylcholine receptors occupied 238 ± ..

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Awarded by National Health and Medical Research Council (Australia)

Funding Acknowledgements

The work presented was supported by a project grant from the National Health and Medical Research Council (Australia;; grant number 570930). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.