Sequence type 131fimH30 and fimH41 subclones amongst Escherichia coil isolates in Australia and New Zealand
Benjamin A Rogers, Paul R Ingram, Naomi Runnegar, Matthew C Pitman, Joshua T Freeman, Eugene Athan, Sally Havers, Hanna E Sidjabat, Earlleen Gunning, Mary De Almeida, Kaylene Styles, David L Paterson
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | ELSEVIER SCIENCE BV | Published : 2015
The clonal composition of Escherichia coli causing extra-intestinal infections includes ST131 and other common uropathogenic clones. Drivers for the spread of these clones and risks for their acquisition have been difficult to define. In this study, molecular epidemiology was combined with clinical data from 182 patients enrolled in a case-control study of community-onset expanded-spectrum cephalosporin-resistant E. coli (ESC-R-EC) in Australia and New Zealand. Genetic analysis included antimicrobial resistance mechanisms, clonality by DiversiLab (rep-PCR) and multilocus sequence typing (MLST), and subtyping of ST131 by identification of polymorphisms in the fimH gene. The clonal composition..View full abstract
The laboratory component of this work was supported by a grant from The Royal Brisbane and Women's Hospital Foundation (Herston, Queensland, Australia). Use of the DiversiLab (R) system for this study was supported by bioMerieux (Australia), who supplied consumables for the DiversiLab system at a discounted cost. BAR was supported by an Australian Postgraduate Award scholarship.