Journal article
Cartilage-specific ablation of XBP1 signaling in mouse results in a chondrodysplasia characterized by reduced chondrocyte proliferation and delayed cartilage maturation and mineralization
TL Cameron, IL Gresshoff, KM Bell, KA Piróg, L Sampurno, CL Hartley, EM Sanford, R Wilson, J Ermann, RP Boot-Handford, LH Glimcher, MD Briggs, JF Bateman
Osteoarthritis and Cartilage | Published : 2015
Abstract
Objective: To investigate the invivo role of the IRE1/XBP1 unfolded protein response (UPR) signaling pathway in cartilage. Design: Xbp1flox/flox.Col2a1-Cre mice (Xbp1CartδEx2), in which XBP1 activity is ablated specifically from cartilage, were analyzed histomorphometrically by Alizarin red/Alcian blue skeletal preparations and X-rays to examine overall bone growth, histological stains to measure growth plate zone length, chondrocyte organization, and mineralization, and immunofluorescence for collagen II, collagen X, and IHH. Bromodeoxyuridine (BrdU) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analyses were used to measure chondrocyte proliferation and cell deat..
View full abstractGrants
Awarded by National Institute of Child Health and Human Development
Funding Acknowledgements
This work was funded by the National Health and Medical Research Council of Australia grant #607398 (JFB), the Victorian Government's Operational Infrastructure Support Program, and NIH Grant HD055601 (LHG).