Journal article

Ion Channel Expression in the Developing Enteric Nervous System

Caroline S Hirst, Jaime PP Foong, Lincon A Stamp, Emily Fegan, Stephan Dent, Edward C Cooper, Alan E Lomax, Colin R Anderson, Joel C Bornstein, Heather M Young, Sonja J McKeown

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2015

Abstract

The enteric nervous system arises from neural crest-derived cells (ENCCs) that migrate caudally along the embryonic gut. The expression of ion channels by ENCCs in embryonic mice was investigated using a PCR-based array, RT-PCR and immunohistochemistry. Many ion channels, including chloride, calcium, potassium and sodium channels were already expressed by ENCCs at E11.5. There was an increase in the expression of numerous ion channel genes between E11.5 and E14.5, which coincides with ENCC migration and the first extension of neurites by enteric neurons. Previous studies have shown that a variety of ion channels regulates neurite extension and migration of many cell types. Pharmacological in..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Australian Research Council


Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT


Funding Acknowledgements

National Health and Medical Research Council of Australia Project grant #1047953 to HY and SMcK, Australian Research Council grant #DP130101596 to JB and HY and National Health and Medical Research Council of Australia Senior Research Fellowship #1002506 to HY, http://www.nhmrc.gov.au/, http://www.arc.gov.au/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.