Journal article

Ultrastructure of microsporogenesis and microgametogenesis in Brachypodium distachyon

Akanksha Sharma, Mohan B Singh, Prem L Bhalla



Brachypodium distachyon has emerged as a model system for forage grass and cereal grain species. Here, we report B. distachyon pollen development at the ultrastructural level. The process of microsporogenesis and microgametogenesis in B. distachyon follows the typical angiosperm pollen development sequence. Pronounced evaginations of the nuclear envelope are observed prior to meiosis, indicating active nucleocytoplasmic exchange processes. The microspore mother cells undergo meiosis and subsequent cytokinesis, forming isobilateral tetrads. Following dissolution of the callose wall and release of free and vacuolated microspores, mitotic divisions lead to the formation of mature, three-celled ..

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Awarded by Australian Research Council

Funding Acknowledgements

We thank Dr. Simon Crawford for technical guidance with electron microscopy and access to Advanced Microscopy Facility, The University of Melbourne. AS also thanks Dr. Martin O'Brien for helping in identifying the stages of anther and pollen development and Dr. Lim Chee Liew for suggesting improvements in the manuscript and helping in organizing the figures during manuscript preparation. Financial support from the Australian Research Council (ARC DPO988972) is also gratefully acknowledged.