Journal article

COMPARISON OF COMPUTER-SIMULATIONS OF THE F-TYPE AND L-TYPE NON-OXIDATIVE HEXOSE-MONOPHOSPHATE SHUNTS WITH P-31-NMR EXPERIMENTAL-DATA FROM HUMAN-ERYTHROCYTES

LM MCINTYRE, DR THORBURN, WA BUBB, PW KUCHEL

EUROPEAN JOURNAL OF BIOCHEMISTRY | SPRINGER VERLAG | Published : 1989

Abstract

Mathematical modelling was used to predict the behaviour of the two most favoured schemes for the operation of the non-oxidative hexose monophosphate shunt (HMS), the F-type and the L-type pathways. The models simulate the time courses of sugar-phosphate concentrations when various substrates are metabolized via each pathway. A 31P-NMR technique, with which to observe time courses of concentrations of sugar phosphates in a human red cell lysate, was developed. The accuracy of each hypothesised scheme was then evaluated by comparing predicted with observed data. The results were more consistent with time courses of sugar-phosphate levels predicted by the F-type (classical) pathway than those ..

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