Journal article

THE RELATIONSHIP BETWEEN GLUCOSE-CONCENTRATION AND RATE OF LACTATE PRODUCTION BY HUMAN-ERYTHROCYTES IN AN OPEN PERFUSION SYSTEM

PW KUCHEL, BE CHAPMAN, VA LOVRIC, JE RAFTOS, IM STEWART, DR THORBURN

BIOCHIMICA ET BIOPHYSICA ACTA | ELSEVIER SCIENCE BV | Published : 1984

Abstract

A thermodynamically open system, based on an assembly of capillaries with semi-permeable walls was constructed in order to study glycolysis in human erythrocytes in high haematocrit suspensions. A phenomenological expression for the rate of lactate production as a function of glucose concentration was obtained. The rate was measured under steady-state conditions with low substrate concentrations (approx. 50 mumol/l). In a corresponding closed system, this concentration of glucose would be exhausted within a few minutes. A mathematical model of the whole system consisted of five differential equations, and involved parameters relating to flow rates, volumes of reaction chambers, the rates of ..

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