Journal article

REGULATION OF THE HUMAN-ERYTHROCYTE HEXOSE-MONOPHOSPHATE SHUNT UNDER CONDITIONS OF OXIDATIVE STRESS - A STUDY USING NMR-SPECTROSCOPY, A KINETIC ISOTOPE EFFECT, A RECONSTITUTED SYSTEM AND COMPUTER-SIMULATION

DR THORBURN, PW KUCHEL

The FEBS Journal | WILEY | Published : 1985

Abstract

The regulation of the hexose monophosphate shunt of human erythrocytes under conditions of oxidative stress has been investigated by monitoring the reduction of oxidised glutathione (GSSG) to reduced glutathione (GSH) in erythrocytes containing high levels of GSSG; 1H NMR and a biochemical assay were used to measure the changes. A reconstituted metabolic system prepared with the purified erythrocyte enzymes was used in conjunction with studies of intact cells and haemolysates to determine the dependence of the rate of GSH production on the activities of hexokinase and glucose-6-phosphate dehydrogenase. Both of these enzymes have previously been claimed to be the rate-limiting step of oxidati..

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