Journal article

THE USE OF ANTICLUSTERIN MONOCLONAL-ANTIBODIES FOR THE COMBINED ASSESSMENT OF HUMAN SPERM MORPHOLOGY AND ACROSOME INTEGRITY

MK OBRYAN, BF MURPHY, DY LIU, GN CLARKE, HWG BAKER

HUMAN REPRODUCTION | OXFORD UNIV PRESS UNITED KINGDOM | Published : 1994

Abstract

Clusterin is an abundant protein in the human male reproductive tract which appears to be produced by the testis, epididymis and the seminal vesicles. Using monoclonal antibodies and an amplified immunoperoxidase technique, we have identified two apparently biochemically distinct forms of clusterin on human spermatozoa. Morphologically abnormal spermatozoa have an extensive surface coating of conventional 80 kDa native clusterin, but this form of clusterin is not detectable on normal spermatozoa. Normal spermatozoa, however, contain within the acrosomal cap a different form of clusterin, reactive with an anticlusterin alpha-chain antibody. Agglutinated spermatozoa, most of which are grossly ..

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University of Melbourne Researchers