Journal article

An Evaluation of a Novel Dual Treponemal/Nontreponemal Point-of-Care Test for Syphilis as a Tool to Distinguish Active From Past Treated Infection

Louise M Causer, John M Kaldor, Damian P Conway, David E Leslie, Ian Denham, Theo Karapanagiotidis, Claire Ryan, Handan Wand, David A Anderson, Peter W Robertson, Anna M McNulty, Basil Donovan, Christopher K Fairley, Rebecca J Guy

Clinical Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2015


BACKGROUND: Most syphilis point-of-care (POC) tests detect treponemal antibodies, which persist after successful treatment. Subsequent POC tests are positive, despite no active infection, and can lead to unnecessary treatment. We evaluated a new POC test, incorporating a nontreponemal component, to distinguish active from past infection. METHODS: Sera stored at 2 Australian laboratories were tested with DPP Screen and Confirm Assay. Treponemal and nontreponemal test lines were compared to corresponding conventional treponemal and nontreponemal reference test results: immunoassays and rapid plasma reagin (RPR), respectively, with RPR quantification by endpoint titration. POC test outcome conc..

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