An Evaluation of a Novel Dual Treponemal/Nontreponemal Point-of-Care Test for Syphilis as a Tool to Distinguish Active From Past Treated Infection
Louise M Causer, John M Kaldor, Damian P Conway, David E Leslie, Ian Denham, Theo Karapanagiotidis, Claire Ryan, Handan Wand, David A Anderson, Peter W Robertson, Anna M McNulty, Basil Donovan, Christopher K Fairley, Rebecca J Guy
Clinical Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2015
BACKGROUND: Most syphilis point-of-care (POC) tests detect treponemal antibodies, which persist after successful treatment. Subsequent POC tests are positive, despite no active infection, and can lead to unnecessary treatment. We evaluated a new POC test, incorporating a nontreponemal component, to distinguish active from past infection. METHODS: Sera stored at 2 Australian laboratories were tested with DPP Screen and Confirm Assay. Treponemal and nontreponemal test lines were compared to corresponding conventional treponemal and nontreponemal reference test results: immunoassays and rapid plasma reagin (RPR), respectively, with RPR quantification by endpoint titration. POC test outcome conc..View full abstract
Awarded by National Health and Medical Research Council, Australia
This work was supported by the National Health and Medical Research Council, Australia (application number 568971).