Journal article

Differential effects of superoxide dismutase and superoxide dismutase/catalase mimetics on human breast cancer cells

Manisha H Shah, Guei-Sheung Liu, Erik W Thompson, Gregory J Dusting, Hitesh M Peshavariya



Reactive oxygen species (ROS) such as superoxide and hydrogen peroxide (H2O2) have been implicated in development and progression of breast cancer. In the present study, we have evaluated the effects of the superoxide dismutase (SOD) mimetic MnTmPyP and the SOD/catalase mimetic EUK 134 on superoxide and H2O2 formation as well as proliferation, adhesion, and migration of MCF-7 and MDA-MB-231 cells. Superoxide and H2O2 production was examined using dihydroethidium and Amplex red assays, respectively. Cell viability and adhesion were measured using a tetrazolium-based MTT assay. Cell proliferation was determined using trypan blue assay. Cell cycle progression was analyzed using flow cytometry. ..

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Awarded by Susan G. Komen Foundation for the Cure, USA

Awarded by National Health and Medical Research Council

Funding Acknowledgements

MHS was supported by postdoctoral Fellowship (KG080837) from the Susan G. Komen Foundation for the Cure, USA. GJD is the recipient of an National Health and Medical Research Council Research Fellowship (#1003113). Authors are thankful to Dr. Rachana Sainger for her extensive reading and suggestions for this manuscript. The Centre for Eye Research Australia, St. Vincent's Institute of Medical Research and O'Brien Institute acknowledge the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.