Journal article

CRYSTALLOGRAPHIC PHASES THROUGH GENETIC-ENGINEERING - EXPERIENCES WITH COLICIN-A

AD TUCKER, D BATY, MW PARKER, F PATTUS, C LAZDUNSKI, D TSERNOGLOU

PROTEIN ENGINEERING | OXFORD UNIV PRESS UNITED KINGDOM | Published : 1989

Abstract

Colicins are antibiotic proteins that kill sensitive Escherichia coli cells. The structure of the pore-forming fragment of colicin A has been solved to 2.5 A resolution using the techniques of X-ray crystallography and genetic engineering. Site-directed mutagenesis was used to construct a number of cysteine-containing mutant proteins, one of which yielded an excellent mercurial derivative. Our experiences suggest strategies for obtaining useful heavy-atom derivatives for protein crystallography using genetic engineering techniques.