Journal article

Autophagy is required for stem cell mobilization by G-CSF

Lucie Leveque-El Mouttie, Therese Vu, Katie E Lineburg, Rachel D Kuns, Frederik O Bagger, Bianca E Teal, Mary Lor, Glen M Boyle, Claudia Bruedigam, Justine D Mintern, Geoffrey R Hill, Kelli PA MacDonald, Steven W Lane

BLOOD | AMER SOC HEMATOLOGY | Published : 2015

Abstract

Granulocyte colony-stimulating factor (G-CSF) is widely used clinically to prevent neutropenia after cytotoxic chemotherapy and to mobilize hematopoietic stem cells (HSCs) for transplantation. Autophagy, a process of cytoplasmic component recycling, maintains cellular homeostasis and protects the cell during periods of metabolic stress or nutrient deprivation. We have observed that G-CSF activates autophagy in neutrophils and HSCs from both mouse and human donors. Furthermore, G-CSF-induced neutrophil and HSC mobilization is impaired in the absence of autophagy. In contrast, autophagy is dispensable for direct HSC mobilization in response to the CXCR4 antagonist AMD3100. Altogether, these da..

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Grants

Awarded by National Health and Medical Research Council (NHMRC)


Funding Acknowledgements

These studies were supported by the University of Queensland International Research Tuition Award and University of Queensland Research Scholarship (L.L.-E.M.), the Novo Nordisk Foundation (F.O.B.), and the National Health and Medical Research Council (NHMRC) (grants 1011787, 1059286, and 1089138). J.D.M. and S.W.L. are Australia Fellows and Queensland Career Development Fellows, G.R.H. is an NHMRC and Queensland Health Senior Clinical Research Fellow, K.P.A.M. is a Cancer Council Queensland Senior Research Fellow, and S.W.L. has received fellowship funding from the Leukaemia Foundation.