Journal article

A ray of venom: Combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii)

Kate Baumann, Nicholas R Casewell, Syed A Ali, Timothy NW Jackson, Irina Vetter, James S Dobson, Scott C Cutmore, Amanda Nouwens, Vincent Lavergne, Bryan G Fry

JOURNAL OF PROTEOMICS | ELSEVIER | Published : 2014

Abstract

UNLABELLED: Fish venoms remain almost completely unstudied despite the large number of species. In part this is due to the inherent nature of fish venoms, in that they are highly sensitive to heat, pH, lyophilisation, storage and repeated freeze-thawing. They are also heavily contaminated with mucus, which makes proteomic study difficult. Here we describe a novel protein-handling protocol to remove mucus contamination, utilising ammonium sulphate and acetone precipitation. We validated this approach using barb venom gland tissue protein extract from the blue-spotted stingray Neotrygon kuhlii. We analysed the protein extract using 1D and 2D gels with LC-MS/MS sequencing. Protein annotation wa..

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Grants

Awarded by UK Natural Environment Research Council


Awarded by Australian Research Council


Awarded by Natural Environment Research Council


Funding Acknowledgements

We thank Lauren Cain and Darren Cook for helping with the images used in Fig. 1. NRC acknowledges support from the UK Natural Environment Research Council (research fellowship #NE/J018678/1) and the University of Queensland, Australia (Travel Award for International Collaborative Research). BGF acknowledges the support of the Australian Research Council (#DP140101085) and the Herman Slade Foundation. SAA was the recipient of postdoctoral fellowship (PDRF Phase II Batch-V) from Higher Education Commission (HEC Islamabad) Pakistan.