Journal article

Conserved amino acids within the N-terminus of the West Nile virus NS4A protein contribute to virus replication, protein stability and membrane proliferation

RL Ambrose, JM Mackenzie



The West Nile virus strain Kunjin virus (WNVKUN) NS4A protein is a multifunctional protein involved in many aspects of the virus life-cycle and is a major component of the WNVKUN replication complex (RC). Previously we identified a conserved region in the C-terminus of NS4A regulating proteolytic processing and RC assembly, and now investigate key conserved residues in the N-terminus of NS4A and their contribution to WNVKUN replication. Mutation of P13 completely ablated replication, whereas, mutation of P48 and D49, near the first transmembrane helix, and G66 within the helix, showed variable defects in replication, virion secretion and membrane proliferation. Intriguingly, the P48 and G66 ..

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University of Melbourne Researchers


Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

We thank Alexander Khromykh, Roy Hall and Paul Young for generously providing the WNV<INF>KUN</INF> infectious clones and replicons, and antibodies. We also acknowledge the assistance of Dr Eric Hanssen at the Advanced Microscopy facility, Bio21 Institute, University of Melbourne. This research was partially supported by a Project Grant (no. 1004619) to J.M. from the National Health and Medical Research Council of Australia.