The Polarity Protein Scribble Regulates Myelination and Remyelination in the Central Nervous System

Andrew A Jarjour; Amanda Boyd; Lukas E Dow; Rebecca K Holloway; Sandra Goebbels; Patrick O Humbert; Anna Williams; Charles Ffrench-Constant

Journal article

The Polarity Protein Scribble Regulates Myelination and Remyelination in the Central Nervous System

Andrew A Jarjour, Amanda Boyd, Lukas E Dow, Rebecca K Holloway, Sandra Goebbels, Patrick O Humbert, Anna Williams, Charles Ffrench-Constant

PLoS Biology | PUBLIC LIBRARY SCIENCE | Published : 2015

DOI: 10.1371/journal.pbio.1002107

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Abstract

The development and regeneration of myelin by oligodendrocytes, the myelin-forming cells of the central nervous system (CNS), requires profound changes in cell shape that lead to myelin sheath initiation and formation. Here, we demonstrate a requirement for the basal polarity complex protein Scribble in CNS myelination and remyelination. Scribble is expressed throughout oligodendroglial development and is up-regulated in mature oligodendrocytes where it is localised to both developing and mature CNS myelin sheaths. Knockdown of Scribble expression in cultured oligodendroglia results in disrupted morphology and myelination initiation. When Scribble expression is conditionally eliminated in th..

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University of Melbourne Researchers

Patrick Humbert Author Biochemistry and Pharmacology

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Grants

Awarded by Medical Research Council

Awarded by Wellcome Trust

Awarded by UK Multiple Sclerosis Society

Awarded by Wellcome Trust Clinical Intermediate Fellowship

Awarded by NH&MRC Biomedical Career Development Award

Funding Acknowledgements

AAJ was supported by a postdoctoral fellowship from the Multiple Sclerosis Society of Canada (mssociety.ca). CffC was supported by funding from the Medical Research Council (Rg61812-Xbag1178, www.mrc.ac.uk), the Wellcome Trust (08900/1Z1/09z, www.wellcome.ac.uk), and the UK Multiple Sclerosis Society (891/08, www.mssociety.org.uk). AW was funded by a Wellcome Trust Clinical Intermediate Fellowship (R40590, www.wellcome.ac.uk). LED was supported by a Cancer Council Victoria Postgraduate Studentship. POH was supported by a NH&MRC Biomedical Career Development Award (566607) and project grant (509336, http://www.nhmrc.gov.au/). The funders had no role in study design, data collection and analysis, a decision to publish, or preparation of the manuscript.