Journal article

TRIMETHOPRIM RESISTANCE TRANSPOSON TN4003 FROM STAPHYLOCOCCUS-AUREUS ENCODES GENES FOR A DIHYDROFOLATE-REDUCTASE AND THYMIDYLATE SYNTHETASE FLANKED BY 3 COPIES OF IS257

DA ROUCH, LJ MESSEROTTI, LSL LOO, CA JACKSON, RA SKURRAY

MOLECULAR MICROBIOLOGY | WILEY | Published : 1989

Abstract

Trimethoprim resistance mediated by the Staphylococcus aureus multi-resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn4003. Nucleotide sequence analysis of Tn4003 revealed it to be 4717 bp in length and to contain three copies of the insertion element IS257 (789-790 bp), the outside two of which are flanked by directly repeated 8-bp target sequences. IS257 has imperfect terminal inverted repeats of 27-28 bp and encodes for a putative transposase with two potential alpha-helix-turn-alpha-helix DNA recognition motifs. IS257 shares sequence similarities with members of the IS15 family of insertion sequences from Gram-nega..

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University of Melbourne Researchers