Journal article
Nontypeable haemophilus influenzae induces sustained lung oxidative stress and protease expression
PT King, R Sharma, K O'Sullivan, S Selemidis, S Lim, N Radhakrishna, C Lo, J Prasad, J Callaghan, P McLaughlin, M Farmer, D Steinfort, B Jennings, J Ngui, BRS Broughton, B Thomas, AT Essilfie, M Hickey, PW Holmes, P Hansbro Show all
Plos One | Published : 2015
Abstract
Nontypeable Haemophilus influenzae (NTHi) is a prevalent bacterium found in a variety of chronic respiratory diseases. The role of this bacterium in the pathogenesis of lung inflammation is not well defined. In this study we examined the effect of NTHi on two important lung inflammatory processes 1), oxidative stress and 2), protease expression. Bronchoalveolar macrophages were obtained from 121 human subjects, blood neutrophils from 15 subjects, and human-lung fibroblast and epithelial cell lines from 16 subjects. Cells were stimulated with NTHi to measure the effect on reactive oxygen species (ROS) production and extracellular trap formation. We also measured the production of the oxidant,..
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Funding Acknowledgements
Supported by Monash University Faculty of Medicine, Nursing and Health Sciences Strategic Grants (to PK) and an Australian Research Council Discovery Grant and an Australian Research Council Future Fellowship Support (to SS).