Conformational Dynamics and Antigenicity in the Disordered Malaria Antigen Merozoite Surface Protein 2
Christopher A MacRaild, Milan Zachrdla, Dean Andrew, Bankala Krishnarjuna, Jiri Novacek, Lukas Zidek, Vladimir Sklenar, Jack S Richards, James G Beeson, Robin F Anders, Raymond S Norton
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2015
Merozoite surface protein 2 (MSP2) of Plasmodium falciparum is an abundant, intrinsically disordered protein that is GPI-anchored to the surface of the invasive blood stage of the malaria parasite. Recombinant MSP2 has been trialled as a component of a malaria vaccine, and is one of several disordered proteins that are candidates for inclusion in vaccines for malaria and other diseases. Nonetheless, little is known about the implications of protein disorder for the development of an effective antibody response. We have therefore undertaken a detailed analysis of the conformational dynamics of the two allelic forms of MSP2 (3D7 and FC27) using NMR spectroscopy. Chemical shifts and NMR relaxat..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
Awarded by project "CEITEC - Central European Institute of Technology" from European Regional Development Fund
This work was supported in part by an Australian National Health and Medical Research Council (NHMRC) project grant (1042520) and by the project "CEITEC - Central European Institute of Technology" (grant CZ.1.05/1.1.00/02.0068) from European Regional Development Fund. JSR, JGB, and RSN acknowledge fellowship support from the NHMRC. The Burnet Institute is supported by funding from the NHMRC Infrastructure for Independent Research Institutes Support Scheme, and Victoria State Government Operational Infrastructure Support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.