Modular Evolution of TnGBSs, a New Family of Integrative and Conjugative Elements Associating Insertion Sequence Transposition, Plasmid Replication, and Conjugation for Their Spreading
Romain Guerillot, Violette Da Cunha, Elisabeth Sauvage, Christiane Bouchier, Philippe Glaser
Journal of Bacteriology | AMER SOC MICROBIOLOGY | Published : 2013
Integrative and conjugative elements (ICEs) have a major impact on gene flow and genome dynamics in bacteria. The ICEs TnGBS1 and TnGBS2, first identified in Streptococcus agalactiae, use a DDE transposase, unlike most characterized ICEs, which depend on a phage-like integrase for their mobility. Here we identified 56 additional TnGBS-related ICEs by systematic genome analysis. Interestingly, all except one are inserted in streptococcal genomes. Sequence comparison of the proteins conserved among these ICEs defined two subtypes related to TnGBS1 or TnGBS2. We showed that both types encode different conjugation modules: a type IV secretion system, a VirD4 coupling protein, and a relaxase and ..View full abstract
Awarded by French National Research Agency
This work was supported by the French National Research Agency (grants ANR-08-GENM-027-001 and 2010-PATH-004-02), the LabEx project IBEID, and the Fondation pour la Recherche Medicale (FRM).