Journal article

Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus

EG Westaway, AA Khromykh, MT Kenney, JM Mackenzie, MK Jones

VIROLOGY | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Published : 1997

Abstract

The subcellular locations in infected Vero cells of Kunjin (KUN) virus core protein C and NS4B were analyzed by immunofluorescence (IF) and by immunoelectron microscopy using monospecific antibodies. Selection of appropriate fixation methods for IF showed that both proteins were associated at all times with perinuclear membranes spreading outward in a reticular pattern and they entered the nucleus late during the latent period. Subsequently NS4B was also dispersed through the nucleoplasm, while C appeared in the nucleolus and the nucleoplasm. These nuclear locations were confirmed by immunogold labeling of cryosections of infected cells at 24 hr postinfection. Labeling of NS4B in cryosection..

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University of Melbourne Researchers