Journal article

ANTIGENIC DETERMINANTS OF INFLUENZA-VIRUS HEMAGGLUTININ .9. THE CARBOHYDRATE SIDE-CHAINS FROM AN ASIAN STRAIN

LE BROWN, CW WARD, DC JACKSON

MOLECULAR IMMUNOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 1982

Abstract

The heavy chain (HA1) of hemagglutinin from the Asian variant A/Japan/305/57 was examined to establish which of the four oligosaccharide units on this polypeptide could cross-react with host antigen. Cyanogen bromide and enzymic digests were fractionated and the resulting peptides screened for their ability to bind to antibodies raised against uninfected chick chorioallantoic membranes. This 324 residue HA1 polypeptide from the Asian strain A/Japan/305/57 is known to contain oligosaccharide units at asparagine residues 11, 23, 285 and at either 164, 165 or both. The peptides carrying the four carbohydrate units have been isolated, the monosaccharide compositions determined and the location o..

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