Journal article

MURINE HELPER LYMPHOCYTE-T RESPONSE TO INFLUENZA-VIRUS - RECOGNITION OF HEMAGGLUTININ BY SUBTYPE-SPECIFIC AND CROSS-REACTIVE T-CELL CLONES

JM KATZ, LE BROWN, RA FFRENCH, DO WHITE

Vaccine | BUTTERWORTH-HEINEMANN LTD | Published : 1985

Abstract

Helper T cel lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. A line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from thes..

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University of Melbourne Researchers