Conference Proceedings

A STUDY OF THE ADVANTAGES AND LIMITATIONS OF IMMUNOBLOTTING PROCEDURES FOR THE DETECTION OF ANTIBODIES AGAINST INFLUENZA-VIRUS

GP KAPAKLISDELIYANNIS, HE DRUMMER, LE BROWN, GA TANNOCK, DC JACKSON

ELECTROPHORESIS | VCH PUBLISHERS INC | Published : 1993

Abstract

An immunoblotting procedure was used to determine the specificity and examine some of the properties of antibodies produced following infection of mice with influenza virus or inoculation with noninfectious material with Alhydrogel or complete Freund's adjuvant. The noninfectious material used was beta-propiolactone-inactivated influenza virus and a preparation (HANA) enriched for the surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). When influenza viral proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions, each of the anti-viral antisera tested exhibited strong binding. Under reducing conditions, however..

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