Journal article

DISTINCT EPITOPES RECOGNIZED BY I-AD-RESTRICTED T-CELL CLONES WITHIN ANTIGENIC SITE-E ON INFLUENZA-VIRUS HEMAGGLUTININ

LE BROWN, RA FFRENCH, JM GAWLER, DC JACKSON, ML DYALLSMITH, EM ANDERS, GW TREGEAR, L DUNCAN, PA UNDERWOOD, DO WHITE

JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 1988

Abstract

A total of 14 I-Ad-restricted helper T-cell clones specific for the hemagglutinin (HA) molecule of influenza virus were isolated from spleens of BALB/c or (BALB/c X C57BL/10)F1 mice immunized with the H3 subtype influenza virus A/Memphis/71 (Mem 71) and from lymph nodes of BALB/c mice primed with purified HA. The specificity of these T-cell clones was assessed in proliferation assays by reactivity with naturally occurring strains of viruses that arose by antigenic drift and contain known amino acid sequence changes in HA and with a panel of monoclonal antibody (MAb)-selected mutants of Mem 71 with single amino acid substitutions in HA. The HA genes of those mutant viruses that failed to stim..

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