Journal article

Distinct properties of the egress-related osmiophilic bodies in male and female gametocytes of the rodent malaria parasite Plasmodium berghei

A Olivieri, L Bertuccini, E Deligianni, B Franke-Fayard, C Currà, I Siden-Kiamos, E Hanssen, F Grasso, F Superti, T Pace, F Fratini, CJ Janse, M Ponzi

Cellular Microbiology | Wiley | Published : 2015


© 2014 John Wiley & Sons Ltd. Summary: Gametogenesis is the earliest event after uptake of malaria parasites by the mosquito vector, with a decisive impact on colonization of the mosquito midgut. This process is triggered by a drop in temperature and contact with mosquito molecules. In a few minutes, male and female gametocytes escape from the host erythrocyte by rupturing the parasitophorous vacuole and the erythrocyte membranes. Electron-dense, oval-shaped organelles, the osmiophilic bodies (OB), have been implicated in the egress of female gametocytes. By comparative electron microscopy and electron tomography analyses combined with immunolocalization experiments, we here define the mor..

View full abstract

University of Melbourne Researchers


Awarded by European Community

Awarded by Italian FLAGSHIP 'InterOmics' project - MIUR

Funding Acknowledgements

We thank Jai Ramesar for technical support. We thank Leonardo Picci for preparation of the immune serum against Pbg377 used in this study. The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 242095 and from the Italian FLAGSHIP 'InterOmics' project (PB.P05) funded by MIUR and coordinated by the CNR. Inga Siden-Kiamos recognizes the support of the OzMalNet fund of the EVIMalaR Network of Excellence for the work carried out at Bio21, University of Melbourne and gratefully acknowledges the support of Ms Vanessa Mollard and Prof. Geoff McFadden. The authors have no conflict of interest.