Lipopolysaccharide Does Not Alter Small Airway Reactivity in Mouse Lung Slices
Chantal Donovan, Simon G Royce, Ross Vlahos, Jane E Bourke
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2015
The bacterial endotoxin, lipopolysaccharide (LPS) has been associated with occupational airway diseases with asthma-like symptoms and in acute exacerbations of COPD. The direct and indirect effects of LPS on small airway reactivity have not been fully elucidated. We tested the hypothesis that both in vitro and in vivo LPS treatment would increase contraction and impair relaxation of mouse small airways. Lung slices were prepared from naïve Balb/C mice and cultured in the absence or presence of LPS (10 μg/ml) for up to 48 h for measurement of TNFα levels in conditioned media. Alternatively, mice were challenged with PBS or LPS in vivo once a day for 4 days for preparation of lung slices or fo..View full abstract
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Awarded by National Health and Medical Research Council (Australia)
Funding was received from the National Health and Medical Research Council (Australia) 1041575, 1027112 http://www.nhmrc.gov.au/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.