The impact of anti-tuberculous antibiotics and corticosteroids on cytokine production in QuantiFERON-TB Gold In Tube assays

V Clifford; C Zufferey; S Germano; N Ryan; D Leslie; A Street; J Denholm; M Tebruegge; N Curtis

Journal article

The impact of anti-tuberculous antibiotics and corticosteroids on cytokine production in QuantiFERON-TB Gold In Tube assays

V Clifford, C Zufferey, S Germano, N Ryan, D Leslie, A Street, J Denholm, M Tebruegge, N Curtis

Tuberculosis | Published : 2015

DOI: 10.1016/j.tube.2015.02.039

Abstract

Introduction The ability to monitor and confirm adequate treatment of latent TB infection (LTBI) would be a major advance. The potential immunomodulatory effects of anti-tuberculous drugs and steroids need to be considered in assessing the utility of cytokine-based assays for this purpose. Methods We determined whether anti-tuberculous antibiotics or dexamethasone affect the production of IFN-γ and other potential cytokine biomarkers (TNF-α, IL-1ra, IL-2, IL-10, IL-13, IP-10, MIP-1β) in the QuantiFERON-TB Gold In-Tube (QFT-IT) assay. Blood from ten adults with LTBI was added to one standard set of QFT-IT tubes and five further sets containing therapeutic concentrations of either isoniazid, r..

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University of Melbourne Researchers

Vanessa Clifford Author

Justin Denholm Author

Nigel Curtis Author

Grants

Funding Acknowledgements

The study was supported by grants from the Murdoch Childrens Research Institute and the John Burge Trust grant 2013; number grant 2013; number; MT is supported by a Clinical Lectureship provided by the U.K. National Institute for Health Research.