Journal article
In vivo multi-modal imaging of experimental autoimmune uveoretinitis in transgenic reporter mice reveals the dynamic nature of inflammatory changes during disease progression
X Chen, JM Kezic, JV Forrester, GL Goldberg, IP Wicks, CC Bernard, PG McMenamin
Journal of Neuroinflammation | BMC | Published : 2015
Abstract
Background: Experimental autoimmune uveoretinitis (EAU) is a widely used experimental animal model of human endogenous posterior uveoretinitis. In the present study, we performed in vivo imaging of the retina in transgenic reporter mice to investigate dynamic changes in exogenous inflammatory cells and endogenous immune cells during the disease process. Methods: Transgenic mice (C57Bl/6 J Cx3 cr1GFP/+, C57Bl/6 N CD11c-eYFP, and C57Bl/6 J LysM-eGFP) were used to visualize the dynamic changes of myeloid-derived cells, putative dendritic cells and neutrophils during EAU. Transgenic mice were monitored with multi-modal fundus imaging camera over five time points following disease induction with ..
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Awarded by National Science Foundation
Funding Acknowledgements
The authors would like to acknowledge Ms Katherine Lim and Dr Jimmy Leong (Monash University, Clayton, VIC, Australia) for masking the clinical fundus images and histological slides for disease grading, and Ms Manpreet Sidhu (Monash University) for assisting with the fundus imaging. The C57Bl/6 CD11c-eYFP and C57Bl/6 J LysM-eGFP mice were kindly provided by Associate Professor Michael Hickey. XC is a recipient of National Health and Medical Research Council (NH& MRC) Dora Lush Biomedical postgraduate scholarship (1039575). GLG is a recipient of the NH& MRC Career Development Fellowship (1034598). CCB is supported by grants from the NH& MRC (1053621), the Victoria/CIRM Joint Project (RMI-01739) and the Department of Industry (AISRF06680), Commonwealth of Australia. PGMcM receives support from the NH& MRC (1069979).