CSF-1 and cell cycle control in macrophages

Abstract

Control of cell proliferation involves a finely interwoven network of positive and negative cell cycle regulators. Signal transduction pathways linking c fms (CSF-1R) to cellular proliferation and differentiation are being explored. Part of the strategy is to use a series of G1 inhibitors to help pinpoint relevant targets. Several inhibitors-8Br-cAMP, interferonγ (IFNγ), INFα/β, lipopolysaccharide (LPS), tumor necrosis factor-α (TNFα), and dimethylamiloride-suppress CSF-1-stimulated proliferation in murine bone marrow-derived macrophages (BMM) even when added in the mid to late-G1 phase of the cell cycle. The down-modulating effects of the inhibitors on the expression of the following cell c..