Coordinate and noncoordinate colony stimulating factor formation by human monocytes

Abstract

The regulation of macrophage colony-stimulating factor (M-CSF) formation by elutriation-purified human monocytes was studied in vitro and compared with that for granulocyte-macrophage CSF (GM-CSF) and granulocyte CSF (G- CSF). The levels of the three CSFs were measured by immunoassay. Lipopolysaccharide (LPS, 100 ng/ml) was able to enhance CSF formation but the levels were significantly influenced by the presence of a cyclooxygenase product(s) in the cultures. In the presence of LPS, both M-CSF and GM-CSF were upregulated by cyclooxygenase inhibition (indomethacin, 10-5 M), while G-CSF was down-regulated. Exogenous prostaglandin E2 (PGE2, 10-7 M) reversed the actions of indomethacin. In LPS-..