Journal article

GM-CSF AND IL-3 STIMULATE DIACYLGLYCEROL GENERATION IN MURINE BONE MARROW-DERIVED MACROPHAGES

N VEIS, JA HAMILTON

Biochemical and Biophysical Research Communications | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Published : 1991

Abstract

In murine bone marrow-derived macrophages, prelabeled with either [3H]myristic acid or [3H]arachidonic acid, the mitogenic colony stimulating factors GM-CSF and IL-3 stimulated a transient increase in [3H]diacylglycerol generation. Maximum [3H]diacylglycerol levels were detected at 10-15 min. The stimulation of [3H]diacylglycerol generation was dependent on the concentration of CSF and correlated with their ability to activate a variety of processes in the macrophage, including DNA synthesis. This is the first report to demonstrate that GM-CSF elevates diacylglycerol levels in macrophages and also to show that diacylglycerol generation may be an important signaling mechanism for IL-3 action...

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