Phorbol ester-stimulated superoxide production by murine bone marrow-derived macrophages requires preexposure to cytokines

Abstract

Murine resident peritoneal macrophages (RPM) generate superoxide (O2) in response to stimulation with PMA or zymosan. Murine bone marrow-derived macrophages (BMM) generate O2 in response to zymosan but not PMA. However, the ability to generate O2 in response to PMA could be induced in BMM by pre-exposing the cells to certain cytokines, including granulocyte-macrophage CSF (GM-CSF), tumor necrosis factor-α (TNF-α), IFN-γ, and, to a lesser extent, IL-1α. Bacterial LPS also induced the ability to respond to PMA. These same agents were also shown to prime RPM for enhanced PMA-induced respiratory burst. In contrast to GM-CSF, CSF-1 did not enhance the ability of BMM or RPM to generate O2 in respo..