Journal article

CONTROL BY IFN-GAMMA AND PGE2 OF TNF-ALPHA AND IL-1 PRODUCTION BY HUMAN-MONOCYTES

PH HART, GA WHITTY, DS PICCOLI, JA HAMILTON

IMMUNOLOGY | BLACKWELL SCIENCE LTD | Published : 1989

Abstract

There have been suggestions that the production of pro-inflammatory mediators by human monocytes in response to interferon-gamma (IFN-gamma) may be controlled by changes in prostaglandins. Therefore we investigated tumour necrosis factor alpha (TNF alpha) and interleukin-1 (IL-1) activities and prostaglandin E2 (PGE2) levels in the supernatants of highly purified human monocytes cultured for 18 hr with recombinant human IFN-gamma. IFN-gamma (100 U/ml) did not stimulate monocytes isolated by counter-current centrifugal elutriation for detectable TNF alpha or IL-1 activities, or PGE2 production. However, IFN-gamma synergistically enhanced lipopolysaccharide (LPS)-induced TNF alpha and IL-1 act..

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