Activation and proliferation signals in murine macrophages: Stimulation of glucose uptake by hemopoietic growth factors and other agents

Abstract

Purified colony‐stimulating factor (CSF‐1) (or macrophage colony stimulating factor [M‐CSF]) stimulated the glucose uptake of murine bone marrow‐derived macrophages (BMM) and resident peritoneal macrophages (RPM) as measured by 3H‐2‐deoxyglucose (2‐DOG) uptake. Similar concentrations of CSF‐1 stimulated the 2‐DOG uptake and DNA synthesis in BMM. Other purified hemopoietic growth factors, granulocyte‐macrophage CSF (GM‐CSF) and interleukin‐3 (IL‐3) (or multi‐CSF), and the tumor promoter, 12‐O‐tetrade‐canoyl‐phorbol‐13‐acetate (TPA), even though differing in their mitogenic capabilities on BMM, were also stimulators of 2‐DOG uptake in BMM and RPM. The nonmitogenic agents, lipopolysaccharide (L..