Journal article
Potentiation of delayed-type hypersensitivity by pertussigen or cyclophosphamide with release of different lymphokines
WA Sewell, PA de Moerloose, JA Hamilton, JW Schrader, IR Mackay, MA Vadas
Immunology | WILEY | Published : 1987
Abstract
The effects on lymphokine production of two agents known to potentiate delayed-type hypersensitivity (DTH), pertussigen (pertussis toxin) (PT) and cyclophosphamide (CY) have been investigated. These two agents were administered to immunized mice. Subsequently, lymph nodes and spleen cells were exposed to specific antigen in vitro. The resulting culture supernatants were assayed for the presence of lymphokines. Only supernatants of cells from the mice given PT contained appreciable quantities of interferon-γ (IFN-γ) and stimulated cells of the monocyte-like WEHI-265 cell line to produce procoagulant activator and plasminogen activator. On the other hand, CY was more effective than PT on the p..
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