The nature of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA)‐stimulated hemopoiesis, colony stimulating factor (CSF) requirement for colony formation, and the effect of TPA on [125I]CSF‐1 binding to macrophages

Abstract

The tumor‐promoting phorbol diester, 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) was found to act both independently of and synergistically with the mononuclear phagocyte specific colony stimulating factor (CSF‐1) to stimulate the formation of macrophage colonies in cultures of mouse bone marrow cells. In contrast, TPA did not synergize with other CSF subclasses that stimulate the formation of eosinophil, eosinophil‐neutrophil, neutrophil, neutrophil‐macrophage, and macrophage colonies, nor with either of the two factors required for megakaryocyte colony formation, megakaryocyte CSF, and megakaryocyte colony potentiator. In serum‐free mouse bone marrow cell cultures TPA retained the ability t..