Journal article

THE NATURE OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE (TPA)-STIMULATED HEMATOPOIESIS, COLONY STIMULATING FACTOR (CSF) REQUIREMENT FOR COLONY FORMATION, AND THE EFFECT OF TPA ON [I-125] CSF-1 BINDING TO MACROPHAGES

LJ GUILBERT, DJ NELSON, JA HAMILTON, N WILLIAMS

JOURNAL OF CELLULAR PHYSIOLOGY | WILEY | Published : 1983

Abstract

The tumor-promoting phorbol diester, 12-O-tetradecanoylphorbol-13-acetate (TPA) was found to act both independently of and synergistically with the mononuclear phagocyte specific colony stimulating factor (CSF-1) to stimulate the formation of macrophage colonies in cultures of mouse bone marrow cells. In contrast, TPA did not synergize with other CSF subclasses that stimulate the formation of eosinophil, eosinophil-neutrophil, neutrophil, neutrophil-macrophage, and macrophage colonies, nor with either of the two factors required for megakaryocyte colony formation, megakaryocyte CSF, and megakaryocyte colony potentiator. In serum-free mouse bone marrow cell cultures TPA retained the ability t..

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