Remote Ischemic Preconditioning (RIPC) Modifies the Plasma Proteome in Children Undergoing Repair of Tetralogy of Fallot: A Randomized Controlled Trial

Michele Hepponstall; Vera Ignjatovic; Steve Binos; Chantal Attard; Vasiliki Karlaftis; Yves d'Udekem; Paul Monagle; Igor E Konstantinov

Journal article

Remote Ischemic Preconditioning (RIPC) Modifies the Plasma Proteome in Children Undergoing Repair of Tetralogy of Fallot: A Randomized Controlled Trial

Michele Hepponstall, Vera Ignjatovic, Steve Binos, Chantal Attard, Vasiliki Karlaftis, Yves d'Udekem, Paul Monagle, Igor E Konstantinov

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2015

DOI: 10.1371/journal.pone.0122778

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Abstract

BACKGROUND: Remote ischemic preconditioning (RIPC) has been applied in paediatric cardiac surgery. We have demonstrated that RIPC induces a proteomic response in plasma of healthy volunteers. We tested the hypothesis that RIPC modifies the proteomic response in children undergoing Tetralogy of Fallot (TOF) repair. METHODS AND RESULTS: Children (n=40) were randomized to RIPC and control groups. Blood was sampled at baseline, after cardiopulmonary bypass (CPB) and 6, 12 and 24h post-CPB. Plasma was analysed by liquid chromatography mass spectrometry (LC-MS) in an untargeted approach. Peptides demonstrating differential expression (p<0.01) were subjected to tandem LC-MS/MS and protein identific..

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University of Melbourne Researchers

Igor Konstantinov Author Paediatrics Royal Children's Hospital

Yves D'Udekem D'Acoz Author Paediatrics Royal Children's Hospital

Paul Monagle Author Paediatrics Royal Children's Hospital

Vera Ignjatovic Author Paediatrics Royal Children's Hospital

Chantal Attard Author Paediatrics Royal Children's Hospital

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Grants

Awarded by National Health and Medical Research Council (NHMRC)

Awarded by NHMRC postgraduate scholarship

Funding Acknowledgements

The project was funded by the National Health and Medical Research Council (NHMRC) grant (#628756) and NHMRC postgraduate scholarship (#1017734). The study was supported by the Victorian Governments's Operational Infrastructure Support Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.