Journal article

Distribution of the orexin-1 receptor ((OXR)-R-1.) in the mouse forebrain and rostral brainstem: A characterisation of OX1R-eGFP mice

Sarah Sulaiman Ch'ng, Andrew J Lawrence

JOURNAL OF CHEMICAL NEUROANATOMY | ELSEVIER SCIENCE BV | Published : 2015

Abstract

We have utilised a transgenic reporter mouse in which green fluorescent protein (GFP) expression is driven by the orexin-1 receptor (OX1R) promoter to systematically map the distribution of OX1R-expressing neurons throughout the mouse forebrain and rostral brainstem. GFP labelling was observed in perikarya and fibres in an extensive range of brain loci encompassing the olfactory and cerebral cortices, dorsal and ventral pallidum, hippocampus, amygdaloid regions, septal areas, thalamic nuclei, hypothalamic nuclei and several brainstem regions, consistent with previous studies of OX1R mRNA expression. This is the first study to systematically characterise the neuroanatomical distribution of OX..

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Grants

Funding Acknowledgements

We thank Dr Danny Winder (Vanderbilt University, USA) and Dr Paul Kenny (Mt Sinai School of Medicine, USA) for the OK<INF>1</INF>R-eGFP founder mice. We also thank Rose Chesworth and Philip Ryan for their assistance with immunohistochemical techniques. This work was supported by project grants from the National Health and Medical Research Council (NHMRC, Australia) of which AJL is a Principal Research Fellow. We acknowledge the Victorian Government's Operational Infrastructure Support Program.