Journal article
Extensive capsule locus variation and large-scale genomic recombination within the Klebsiella pneumoniae clonal group 258
KL Wyres, C Gorrie, DJ Edwards, HFL Wertheim, LY Hsu, N Van Kinh, R Zadoks, S Baker, KE Holt
Genome Biology and Evolution | Published : 2015
DOI: 10.1093/gbe/evv062
Open access
Abstract
Klebsiella pneumoniae clonal group (CG) 258, comprising sequence types (STs) 258, 11, and closely related variants, is associated with dissemination of the K.pneumoniae carbapenemase (KPC). Hospitaloutbreaks of KPC CG258infections have been observed globally and are very difficult to treat. As a consequence, there is renewed interest in alternative infection control measures such as vaccines and phage or depolymerase treatments targeting the K. pneumoniae polysaccharide capsule. To date, 78 immunologically distinct capsule variants have been described in K. pneumoniae. Previous investigations of ST258 and a small number of closely related strains suggested that capsular variation was limited..
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Awarded by Wellcome Trust
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia (Fellowship #628930) to K.E.H., the Victorian Life Sciences Computation Initiative (#VR0082) and the Wellcome Trust and the Royal Society (Sir Henry Dale Fellowship #100087/Z/12/Z) to S.B. The authors thank Trinh Dao Tuyet (National Hospital for Tropical Diseases, Vietnam), Tse Hsien Koh (Singapore General Hospital, Singapore), Mark Thomas and Peter Ostrum (Countryside Veterinary Clinic, LLP, USA) for the provision of bacterial isolates. They also thank the sequencing teams at the Wellcome Trust Sanger Institute for genome sequencing of eight isolates (funded by Wellcome Trust grant #098051 to Wellcome Trust Sanger Institute) and the Microbiological Diagnostic Unit (University of Melbourne, Australia) for sequencing of the KpMDU1 isolate.