Journal article

RELEASE AND PROPERTIES OF ENDOTHELIUM-DERIVED RELAXING FACTOR (EDRF) FROM ENDOTHELIAL-CELLS IN CULTURE

TM COCKS, JA ANGUS, JH CAMPBELL, GR CAMPBELL

JOURNAL OF CELLULAR PHYSIOLOGY | WILEY-LISS | Published : 1985

Abstract

Cultured bovine endothelial cells were seeded onto the intimal surface of endothelium-denuded rings of canine coronary artery. These rings did not previously relax to acetylcholine, substance P, bradykinin, and A23187. After seeding, the same rings relaxed to bradykinin and A23187, but not to acetycholine or substance P. Indomethacin pretreatment did not affect these responses. Cells from the same source were then grown to confluence on microcarrier beads, poured into small columns, and perfused with Krebs' solution. The perfusate from the columns was bioassayed on endothelium-denuded rings of coronary artery from either the dog or pig. Challenge of the column in the presence of indomethacin..

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