Journal article

Analysis of the golgi apparatus in Arabidopsis seed coat cells during polarized secretion of pectin-rich mucilage

Robin E Young, Heather E McFarlane, Michael G Hahn, Tamara L Western, George W Haughn, A Lacey Samuels

PLANT CELL | AMER SOC PLANT BIOLOGISTS | Published : 2008

Abstract

Differentiation of the Arabidopsis thaliana seed coat cells includes a secretory phase where large amounts of pectinaceous mucilage are deposited to a specific domain of the cell wall. During this phase, Golgi stacks had cisternae with swollen margins and trans-Golgi networks consisting of interconnected vesicular clusters. The proportion of Golgi stacks producing mucilage was determined by immunogold labeling and transmission electron microscopy using an antimucilage antibody, CCRC-M36. The large percentage of stacks found to contain mucilage supports a model where all Golgi stacks produce mucilage synchronously, rather than having a subset of specialist Golgi producing pectin product. Init..

View full abstract

University of Melbourne Researchers